Accelerated differentiation of human induced pluripotent stem cells into regionally specific dorsal and ventral spinal neural progenitor cells for application in spinal cord therapeutics

Spinal cord injury can attenuate both motor and sensory function with minimal potential for full recovery. Research utilizing human induced pluripotent stem cell (hiPSC) -derived spinal types in vivo remodeling neuromodulation after has grown substantially recent years. However, the majority of protocols differentiation neurons are lengthy, lack appropriate dorsoventral or rostrocaudal specification, not typically replicated more than one line. Furthermore, most researchers currently utilize hiPSC-derived transplantation injury, very little exploration neuron transplantation. The studies that populations may be due part to relative scarcity dorsal horn protocols. Building upon our previously published work demonstrated rapid establishment a primitive ectoderm population from hiPSCs, we describe here production diverse ventral progenitor cells. Our creates novel system allowing differentiated same intermediate population, making it possible construct domains while decreasing variability. This technology used tandem biomaterials pharmacology improve increasing neuroregeneration.